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Due to moncler outlet online the diffraction phenomena, each 2D image, also called optical section, includes considerable out of focus light originating from regions of the specimen above and below the focal plane. Image deconvolution uses information describing how the microscope produces the image (forward model) as the basis of a mathematical transformation that reassigns the out of focus light to the points of origin.Later, many new optical methods have been proposed to remove out of focus light and to generate directly true optical sections. Without pretending to be exhaustive, we mention confocal laser scanning microscopy (CLSM)2,3, two photon excitation microscopy (TPEM)2,4 and selective plane illumination microscopy (SPIM)5,6.
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